- Autor
- Benítez-Mateos, Ana
- Nidetzky, Bernd
- Bolivar, Juan Manuel
- Lòpez-Gallego, Fernando
- TitelSingle-Particle Studies to Advance the Characterization of Heterogeneous Biocatalysts
- Datei
- DOI10.1002/cctc.201701590
- Erschienen inChemCatChem
- Band10
- Erscheinungsjahr2018
- Heft4
- Seiten654-665
- LicenceCC BY 4.0
- ISSN1867-3899
- Download Statistik83
- Peer ReviewJa
- AbstractImmobilized enzymes have been widely exploited because they work as heterogeneous biocatalysts, allowing their recov- ery and reutilization and easing the downstream processing once the chemical reactions are completed. Unfortunately, we suffer a lack of analytical methods to characterize those heter- ogeneous biocatalysts at microscopic and molecular levels with spatio-temporal resolution, which limits their design and optimization. Single-particle studies are vital to optimize the performance of immobilized enzymes in micro/nanoscopic environments. In this Concept article, we review different ana- lytical techniques that address single-particle studies to image the spatial distribution of the enzymes across the solid surfa- ces, the sub-particle substrate diffusion, the structural integrity and mobility of the immobilized enzymes inside the solid parti- cles, and the pH and O2 internal gradients. From our view, such sub-particle information elicited from single-particle anal- ysis is paramount for the design and fabrication of optimal heterogeneous biocatalyst.
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